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11.
RY Poon K Yamashita JP Adamczewski T Hunt J Shuttleworth 《Canadian Metallurgical Quarterly》1993,12(8):3123-3132
Activation of the cyclin-dependent protein kinases p34cdc2 and p33cdk2 requires binding with a cyclin partner and phosphorylation on the first threonine residue in the sequence THEVVTLWYRAPE. We present evidence that this threonine residue, number 160 in p33cdk2, can be specifically phosphorylated by a cdc2-related protein kinase from Xenopus oocytes called p40MO15. Binding to cyclin A and phosphorylation of this threonine are both required to activate fully the histone H1 kinase activity of p33cdk2. In cell extracts, a portion of p40MO15 is found in a high molecular weight complex that is considerably more active than a lower molecular weight form. Wild-type MO15 protein expressed in bacteria does not possess kinase activity, but acquires p33cdk2-T160 kinase activity after incubation with cell extract and ATP. We conclude that p40MO15 corresponds to CAK (cdc2/cdk2 activating kinase) and speculate that, like p33cdk2 and p34cdc2, p40MO15 requires activation by phosphorylation and association with a companion subunit. 相似文献
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13.
Yoshio Yamashita Hideyuki Jinbo Ryuji Kawazu Takateru Asano Hiroshi Umehara 《Polymer Engineering and Science》1991,31(12):855-859
LMR-UV (“low molecular weight resist for uv lithography”), a naphthoquinone-diazide sulfonic acid ester of a novolak resin, is a negative working resist. The mechanism of insolubilization of LMR-UV is based on the facts that the naphthoquinone-diazide moiety is decomposed to indenecarboxylic acid (polar compound) by photolysis upon UV irradiation and that the irradiated resist film insolubilizes in a non-polar developer. LMR-UV reliably forms 0.6 μm lines and spaces over a reflective substrate with steps by using a g-line stepper having a 0.35 NA lens. 0.6 μm-wide aluminum patterns over topography are obtained by use of g-line exposure and reactive ion etching. By use of an i-line aligner (NA = 0.42), LMR-UV resolves 0.25 μm space patterns with overhang profiles. The profiles are due to the large absorption coefficient of 3.8 μm?1. 0.3 μm wide aluminum patterns are formed by i-line exposure and lift-off metallization. 相似文献
14.
Y Yamashita M Harada T Sawada M Takahashi K Miyazaki H Okamura 《Canadian Metallurgical Quarterly》1993,186(2):495-501
Dynamic magnetic resonance (MR) imaging was performed on a 1.5-T superconducting unit for evaluation of 26 stage I endometrial carcinomas. To establish the appearance of the normal uterus, 27 normal uteri were also evaluated. After rapid injection of gadopentetate dimeglumine, dynamic images were obtained every 30 seconds with the spin-echo technique in the sagittal plane. On dynamic studies of endometrial carcinoma, the tumor-myometrial contrast was marked at 120 seconds after administration of gadopentetate dimeglumine (contrast-to-noise ratio [C/N], 26.0). The tumor-myometrial contrast on the dynamic study was more marked than that on postcontrast T1-weighted images (C/N, 10.0) and on T2-weighted images (C/N, 2.14). Dynamic and postcontrast MR images were superior in enabling differentiation of viable tumors from necrosis or residual secretion in the endometrial cavity. In the evaluation of presence of tumor and myometrial invasion, the accuracy of T2-weighted imaging and dynamic imaging was 67.9% and 84.9%, respectively. 相似文献
15.
Ito M. Miki T. Hosotani S. Kumamoto T. Yamashita Y. Kijima M. Okuda T. Okada K. 《Solid-State Circuits, IEEE Journal of》1994,29(12):1531-1536
A 10 bit CMOS A/D converter with 3 V power supply has been developed for being integrated into system VLSI's. In this A/D converter, redundant binary encoders named “twin encoders” enhance tolerance to substrate noise, together with employing differential amplifiers in comparators. The bias circuit using a replica of the amplifier is developed for biasing differential comparators with 3 V power supply. Subranging architecture along with a multilevel tree decoding structure improves dynamic performance of the ADC at 3 V power supply. The A/D converter is fabricated in double-polysilicon, double-metal, 0.8 μm CMOS technology. The experimental results show that the ADC operates at 20 MS/s and the twin encoders suppress the influence of substrate noise effectively. This ADC has a single power supply of 3 V, and dissipates 135 mW at 20 MS/s operation 相似文献
16.
The propagation and dispersion characteristics of picosecond electrical pulses in a suspended coplanar waveguide (SCPW) are investigated, and it is shown that the SCPW is a very promising transmission structure for ultrashort pulses. Numerical results for the modal dispersion of the SCPW are presented and compared to those of the conventional CPW, and a field-coupling theory is used to explain the evolution in the dispersion behavior. An evaluation based on the numerical analysis shows that a SCPW with properly controlled dispersion can exhibit a five times improvement in pulse transmission capability compared to the conventional CPW. Both computer simulations and experimental measurements show a substantial suppression in pulse distortion as well, compared to conventional CPWs 相似文献
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18.
Kazuhiro Arai Yoichi Yamashita Riichiro Mizoguchi 《Expert systems with applications》1992,5(3-4):219-232
System monitoring and diagnosis through signal interpretation are important tasks in many engineering fields. Although monitoring or diagnosis expert systems help human experts a lot, it is not so easy to build a signal interpretation expert system. We have developed two expert systems for speech signal processing. The close analysis of the two systems revealed common functions and modules applicable to wide range of signal interpretation problems. On the basis of the experience, we have developed a shell for signal interpretation expert systems named SSI. In order to evaluate SSI, we also developed two expert systems by using it and obtained satisfactory results. This paper describes the design philosophy and architecture of SSI together with some examples. 相似文献
19.
Bacterial cells respond to monoamine compounds, such as tyramine, dopamine, octopamine, or norepinephrine, and induce the syntheses of tyramine oxidase encoded by tynA and monoamine oxidase encoded by maoA. These monoamine compounds also derepress the synthesis of atsA-specified arylsulfatase that is repressed by sulfur compounds. These complex mechanisms of regulons regulated by monoamine and sulfur compounds has been analyzed by cloning and characterization of genes that are involved in the repression and derepression of the synthesis of arylsulfatase. The atsA gene forms an operon with the atsB gene, which encodes an activator of the expression of atsA. The negative regulator gene for arylsulfatase was found to code for dihydrofolate reductase (folA). The maoA gene forms an operon with the maoC gene, which has similarity to a dehydrogenase involved in the tyramine metabolism. The moaF gene encoding a 30-kDa protein, which is induced by tyramine, also forms an operon with the moaE gene. Finally, the moaR gene, which is induced by monoamine, was found to play a central role in the positive regulation of the expression of the monoamine regulon (moa) including the atsBA, maoCA, moaEF, and tyn operons. The moaR expression is subject to autogenous regulation and to cAMP-CRP control. The MoaR protein has a helix-turn-helix motif in its C terminus. Thus, the MoaR protein probably regulates the operons by binding to the regulatory region of the moa regulon. 相似文献
20.
The effect of magnetic field on OH radical distribution in a hydrogen-oxygen diffusion flame was experimentally and numerically investigated to explore the possibility of combustion control by magnetic force. In experiments, a coaxial type of burner was set between the magnet pieces. Two-dimensional (2D) cross-section distributions of OH* chemiluminescence intensity and OH fluorescence intensity were obtained with spectroscopic techniques using a CCD camera and a Planar Laser-Induced Fluorescence (PLIF) system, respectively. It was clearly seen that the high-density regions of OH* and OH radicals axisymmetrically migrated toward the central axis of the flame due to the influence of the magnetic field. In numerical simulations, such a phenomenon was qualitatively reproduced by solving the equations of reactive gas dynamics and magnetism. As a result, it was found that the magnetic force does not directly and selectively induce the diffusion velocity (the relative velocity) of OH itself. Alternatively, the magnetic force acting on O2, whose mass density and magnetic susceptibility are much larger than those of other chemical species, causes the change in the mean velocity (the mass-average velocity) of mixture gas to transport the OH radical distribution indirectly and passively. 相似文献